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a549  (ATCC)


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    ATCC a549
    A549, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 9086 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+lung+epithelial+cell+line+a549/A549/custom%40crm-ccl-185%4010%2E64898%2F2026%2E08%2E02%2E742305
    Average 99 stars, based on 9086 article reviews
    a549 - by Bioz Stars, 2026-09
    99/100 stars

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    Cell Culture:

    Article Title: Phage-derived depolymerase targeting the K27 capsule impairs Klebsiella pneumoniae virulence, biofilm formation, and promotes immune clearance
    Article Snippet: .. The human lung epithelial cell line A549 (ATCC CCL-185) was cultured in F-12K medium (BioWest, Nuaille, France) supplemented with 10% foetal bovine serum (Cytogen, Zgierz, Poland) at 37 °C in 5% CO 2 . ..

    Article Title: The Therapeutic Administration of Lactobacillus brevis ZG2488 Suppresses Influenza A Virus Replication Through a Viability-Dependent Host Transcriptional Modulation Mechanism.
    Article Snippet: .. The human lung epithelial cell line A549 was obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA) and cultured in Dulbecco’s Modified Eagle Medium (DMEM, Gibco, Grand Island, NY, USA) supplemented with 10% fetal bovine serum (FBS, Gibco, Grand Island, NY, USA) and 1% penicillin–streptomycin at 37 ◦C in a humidified incubator with 5% CO2. ..

    Article Title: The Therapeutic Administration of Lactobacillus brevis ZG2488 Suppresses Influenza A Virus Replication Through a Viability-Dependent Host Transcriptional Modulation Mechanism
    Article Snippet: .. The human lung epithelial cell line A549 was obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA) and cultured in Dulbecco’s Modified Eagle Medium (DMEM, Gibco, Grand Island, NY, USA) supplemented with 10% fetal bovine serum (FBS, Gibco, Grand Island, NY, USA) and 1% penicillin–streptomycin at 37 °C in a humidified incubator with 5% CO 2 . ..

    Article Title: Antimicrobial Activity of Aurisin A Against Streptococcus suis and Its Protective Effect on Epithelial Cells.
    Article Snippet: S. suis was cultured in brain heart infusion broth (BHI) (BHI; HiMedia Laboratories, Mumbai, India) or plated on sheep blood agar (Biomedia, Bangkok, Thailand) at 37 ◦C under 5% CO2. .. The human lung epithelial cell line (A549) was purchased from the American Type Culture Collection (ATCC, Gaithersburg, MD, USA) and cultured in RPMI 1640 (Gibco; Thermo Fisher Scientific, Inc.; Grand Island, NY, USA) supplemented with 10% inactivated fetal bovine serum (FBS) (Gibco; Thermo Fisher Scientific, Inc.; Paisley, UK) 100 units/mL penicillin, and 100 μg/mL streptomycin (Sigma; Grand Island, NY, USA) at 37 ◦C under 5% CO2. ..

    Generated:

    Article Title: Molecular Representation and Closed-Loop Validation for Toxicity Assessment of Organic Compounds in Ambient Air PM 2.5
    Article Snippet: .. To validate the toxicity predictions generated by ML models, in vitro assays were performed with human lung epithelial cell line A549 obtained from the American Type Culture Collection (ATCC). ..

    In Vitro:

    Article Title: Molecular Representation and Closed-Loop Validation for Toxicity Assessment of Organic Compounds in Ambient Air PM 2.5
    Article Snippet: .. To validate the toxicity predictions generated by ML models, in vitro assays were performed with human lung epithelial cell line A549 obtained from the American Type Culture Collection (ATCC). ..

    Modification:

    Article Title: The Therapeutic Administration of Lactobacillus brevis ZG2488 Suppresses Influenza A Virus Replication Through a Viability-Dependent Host Transcriptional Modulation Mechanism.
    Article Snippet: .. The human lung epithelial cell line A549 was obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA) and cultured in Dulbecco’s Modified Eagle Medium (DMEM, Gibco, Grand Island, NY, USA) supplemented with 10% fetal bovine serum (FBS, Gibco, Grand Island, NY, USA) and 1% penicillin–streptomycin at 37 ◦C in a humidified incubator with 5% CO2. ..

    Article Title: The Therapeutic Administration of Lactobacillus brevis ZG2488 Suppresses Influenza A Virus Replication Through a Viability-Dependent Host Transcriptional Modulation Mechanism
    Article Snippet: .. The human lung epithelial cell line A549 was obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA) and cultured in Dulbecco’s Modified Eagle Medium (DMEM, Gibco, Grand Island, NY, USA) supplemented with 10% fetal bovine serum (FBS, Gibco, Grand Island, NY, USA) and 1% penicillin–streptomycin at 37 °C in a humidified incubator with 5% CO 2 . ..



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    Netilmicin sulfate alone and in combination exerted protective effects on an infected <t>A549</t> cell model (A) Cytotoxic effects of the drugs on A549 cells. (B) Protective effects of different concentrations of NETS on A549 cells. The early interventions on the abscissa reflect the incubation of A549 cells with the drug for 2 h before B. pseudomallei HNBP001 infection. Late intervention was defined as the introduction of the drug to A549 cells 2 h after infection with B. pseudomallei HNBP001 . The ordinate is the survival rate of the infected A549 cells. NC: the blank negative control group of fresh 10% DMEM. Bp: infected A549 cell group. Bp+30 μM NETS: The infected A549 cells were treated with 30 μM NETS; the other conditions were the same, but different concentrations of NETS were used. (C) Protective effects of several drugs on infected A549 cells; the ordinate is the survival rate of infected A549 cells. NC, blank negative control group of fresh 10% DMEM. Bp: infected A549 cell group. Other: The infected A549 cells were treated with drugs (including SXT, GM, NETS, CAZ, and NETS+CAZ). (D) Number of intracellular bacteria in infected A549 cells after drug treatment; the ordinate represents the number of bacteria in infected A549 cells. NC is the blank negative control group of fresh 10% DMEM. Bp: infected A549 cell group. Drug group: 2 × MIC drug was used to treat infected A549 cells. All drugs, including SXT, GM, NETS, CAZ, and NETS+CAZ. (∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001; ns, no significant difference. Data are represented as mean ± SEM.).
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    Image Search Results


    Netilmicin sulfate alone and in combination exerted protective effects on an infected A549 cell model (A) Cytotoxic effects of the drugs on A549 cells. (B) Protective effects of different concentrations of NETS on A549 cells. The early interventions on the abscissa reflect the incubation of A549 cells with the drug for 2 h before B. pseudomallei HNBP001 infection. Late intervention was defined as the introduction of the drug to A549 cells 2 h after infection with B. pseudomallei HNBP001 . The ordinate is the survival rate of the infected A549 cells. NC: the blank negative control group of fresh 10% DMEM. Bp: infected A549 cell group. Bp+30 μM NETS: The infected A549 cells were treated with 30 μM NETS; the other conditions were the same, but different concentrations of NETS were used. (C) Protective effects of several drugs on infected A549 cells; the ordinate is the survival rate of infected A549 cells. NC, blank negative control group of fresh 10% DMEM. Bp: infected A549 cell group. Other: The infected A549 cells were treated with drugs (including SXT, GM, NETS, CAZ, and NETS+CAZ). (D) Number of intracellular bacteria in infected A549 cells after drug treatment; the ordinate represents the number of bacteria in infected A549 cells. NC is the blank negative control group of fresh 10% DMEM. Bp: infected A549 cell group. Drug group: 2 × MIC drug was used to treat infected A549 cells. All drugs, including SXT, GM, NETS, CAZ, and NETS+CAZ. (∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001; ns, no significant difference. Data are represented as mean ± SEM.).

    Journal: iScience

    Article Title: Drug screening to identify compounds to eliminate Burkholderia pseudomallei through Hcp protein

    doi: 10.1016/j.isci.2026.115367

    Figure Lengend Snippet: Netilmicin sulfate alone and in combination exerted protective effects on an infected A549 cell model (A) Cytotoxic effects of the drugs on A549 cells. (B) Protective effects of different concentrations of NETS on A549 cells. The early interventions on the abscissa reflect the incubation of A549 cells with the drug for 2 h before B. pseudomallei HNBP001 infection. Late intervention was defined as the introduction of the drug to A549 cells 2 h after infection with B. pseudomallei HNBP001 . The ordinate is the survival rate of the infected A549 cells. NC: the blank negative control group of fresh 10% DMEM. Bp: infected A549 cell group. Bp+30 μM NETS: The infected A549 cells were treated with 30 μM NETS; the other conditions were the same, but different concentrations of NETS were used. (C) Protective effects of several drugs on infected A549 cells; the ordinate is the survival rate of infected A549 cells. NC, blank negative control group of fresh 10% DMEM. Bp: infected A549 cell group. Other: The infected A549 cells were treated with drugs (including SXT, GM, NETS, CAZ, and NETS+CAZ). (D) Number of intracellular bacteria in infected A549 cells after drug treatment; the ordinate represents the number of bacteria in infected A549 cells. NC is the blank negative control group of fresh 10% DMEM. Bp: infected A549 cell group. Drug group: 2 × MIC drug was used to treat infected A549 cells. All drugs, including SXT, GM, NETS, CAZ, and NETS+CAZ. (∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001; ns, no significant difference. Data are represented as mean ± SEM.).

    Article Snippet: The A549 human lung carcinoma epithelial cell line was purchased from Procell (Wuhan, China; Cat# CL-0016).

    Techniques: Infection, Incubation, Negative Control, Bacteria